Astrocytes: Recognizing of featured genes and Neural Stem Cells

International Conference on Cell and Gene Therapy
Date: March 20-21, 2019
Venue: London, UK
Link: http://genetherapy.alliedacademies.com/

Neural stem cells (NSCs) have distinctive biological characteristics like continuous proliferation and multipotential differentiation, providing an attainable methodology for the restoration of central system nervosum (CNS) operate when injury or malady. NSCs and astrocytes share several similar biological properties together with cell morphology and molecular expression and might trans-differentiate into one another underneath bound conditions. However, characteristic genes specifically expressed by NSCs haven't been well.

To provide insights into the characteristic expression of NSCs, bioinformatics analysis of 2 microarrays of mouse NSCs and astrocytes was performed. Compared to astrocytes, the differentially expressed genes (DEGs) in NSCs were known and annotated by GO, KEGG and GSEA analysis, severally.

                                           

Then key genes were screened by protein-protein interaction (PPI) network and modules analysis and were verified victimization multiple high-throughput sequencing resources. Finally, the expression distinction between the 2 cell sorts was confirmed by period Quantitative PCR (qPCR), western blotting and chemical science analysis. In the gift study, 282 and 250 NSC-enriched genes from 2 microarrays were known and annotated severally, and also the seventy-seven overlapping DEGs were then hand-picked.

From the PPI network, twenty-four key genes in 3 modules were screened out. significantly, sequencing information of tissues showed that these twenty-four key genes cared-for be extremely expressed in NSCs compared with astrocytes. what is more, qPCR and western blot analysis of cultivated NSCs and astrocytes showed 2 genes (KIF2C and TOP2A) weren't solely differentially expressed in polymer level however conjointly at the supermolecule level? significantly, the NSC-specific genes KIF2C and TOP2A were valid by assay in vivo.

In gift study, we tend to know a pair of hub genes (KIF2C and TOP2A) that may function potential biomarkers for characteristic NSCs from astrocytes, contributive to our comprehensive understanding of the biological properties and functions of NSCs.